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biohydrogen 1

butyric acid production 1

capillary electrophoresis 1

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Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical

Hanjiang Lai, Chen Huang, Jian Cai, Julian Ye, Jun She, Yi Zheng, Liqian Wang, Yelin Wei, Weijia Fang, Xianjun Wang, Yi-Wei Tang, Yun Luo, Dazhi Jin

Frontiers of Medicine 2018, Volume 12, Issue 2,   Pages 196-205 doi: 10.1007/s11684-017-0560-5

Abstract: polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridiumdifficile genes, including tpi, tcdA, tcdB, cdtAdeletion in tcdC for simultaneous detection and characterization of toxigenic C. difficilewas performed on 354 consecutively collected stool specimens from patients with suspected C. difficileThe toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens

Keywords： Clostridium difficile     multiplex PCR     capillary electrophoresis     detection     characterization    

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Molecular characterization and fermentative hydrogen production of a wild anaerobe in clostridium genus

LI Yongfeng, REN Nanqi, LI Jianzheng, LI Peng, YANG Chuanping

Frontiers in Energy 2007, Volume 1, Issue 4,   Pages 403-407 doi: 10.1007/s11708-007-0058-2

Abstract: Anaerobic process of biohydrogen production is developed in this paper. The isolation and identification of high efficient biohydrogen production anaerobic bacteria are the important foundations for the fermented biohydrogen produc

Keywords： fermented biohydrogen     high     important     identification     biohydrogen    

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High production of butyric acid by

Chao Ma,Jianfa Ou,Matthew Miller,Sarah McFann,Xiaoguang (Margaret) Liu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 369-375 doi: 10.1007/s11705-015-1525-3

Abstract: The objective of this study was to improve the production of butyric acid by process optimization using the metabolically engineered mutant of (PAK-Em). First, the free-cell fermentation at pH 6.0 produced butyric acid with concentration of 38.44 g/L and yield of 0.42 g/g. Second, the immobilized-cell fermentations using fibrous-bed bioreactor (FBB) were run at pHs of 5.0, 5.5, 6.0, 6.5 and 7.0 to optimize fermentation process and improve the butyric acid production. It was found that the highest titer of butyric acid, 63.02 g/L, was achieved at pH 6.5. Finally, the metabolic flux balance analysis was performed to investigate the carbon rebalance in . The results show both gene manipulation and fermentation pH change redistribute carbon between biomass, acetic acid and butyric acid. This study demonstrated that high butyric acid production could be obtained by integrating metabolic engineering and fermentation process optimization.

Keywords： Clostridium tyrobutyricum     butyric acid production     fermentation     mutant     pH     flux balance analysis    

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Title Author Date Type Operation

Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical

Hanjiang Lai, Chen Huang, Jian Cai, Julian Ye, Jun She, Yi Zheng, Liqian Wang, Yelin Wei, Weijia Fang, Xianjun Wang, Yi-Wei Tang, Yun Luo, Dazhi Jin

Journal Article

Molecular characterization and fermentative hydrogen production of a wild anaerobe in clostridium genus

LI Yongfeng, REN Nanqi, LI Jianzheng, LI Peng, YANG Chuanping

Journal Article

High production of butyric acid by

Chao Ma,Jianfa Ou,Matthew Miller,Sarah McFann,Xiaoguang (Margaret) Liu

Journal Article